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The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate
10 g/L Glycerol 315
75 gram powdered medium in 1 litre distilled water
By omitting Folic acid and Riboflavin from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium
LBM-Agar Miller Size:1000g The absence of phosphate (KH2PO4)LBM Agar Low Salt In LBM media Mg2 is added to enhance absorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Agar 15 Final pH 7. 0 0. 2 at 25C Suspend 41 gram powdered medium in 1 litre distilled water Store dry at room temperature
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